Abstract: Objective　To explore the pathogen serotype distribution and drug resistance characteristics of invasive Salmonella infection in children. Method　The clinical characteristics, serotype distribution and drug sensitivity of 66 children with invasive Salmonella infection confirmed by blood or pus culture from January 2012 to December 2015 were retrospectively analyzed. Results　Among 66 children (48 males and 18 females) included, there were 60 hospitalized and 6 outpatient children, and 43 were under 2 years old. Strains were mainly found from May to October (80.30%, 53/66). The main clinical manifestations of hospitalized children were fever (55 cases, 91.67%), abdominal pain and / or diarrhea (32 cases, 53.33%), anemia (36 cases, 60.00%), cough (28 cases, 46.67%), hepatomegaly (24 cases, 40.00%) and splenomegaly (12 cases, 20.00%). Among all the children, 45 (68.18%) had increased white blood cell counts, 56 (84.85%) had decreased proportion of eosinophils (eosinophils disappeared in 15 cases), and 51 (77.27%) had increased level of hypersensitive C-reactive protein. Salmonella serotypes were mainly group D and group B, accounting for 39.39% (26 cases) and 33.33% (22 cases) respectively. The invasive Salmonella strains resistant to imipenem and ertapenem were not detected, and the sensitivities of the detected strains to cefepime, ceftazidime, ceftriaxone and aztreonam were all >70.00%. Conclusion　Invasive Salmonella infection is most common in children under 2 years of age, with prevalence from May to October. Salmonella serotypes were mainly group D and group B, and the clinical manifestations are diverse. Antibiotic treatment should be carefully selected in combination with serotype and drug susceptibility results.