Abstract: Objective　To explore the value of left ventricular myocardial global longitudinal strain (GLS) obtained by two-dimensional speckle tracking echocardiography (STE) in the assessment of acute myocardial injury in children with Kawasaki disease (KD) and its relationship with c-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Method The clinical data of 68 hospitalized children with acute KD from January 2010 to January 2017 were retrospectively analyzed. According to the presence or absence of coronary artery injury, KD children were divided into non-coronary artery injury group (53 cases) and coronary artery injury group (15 cases). The Simpon method was used to measure the left ventricular ejection fraction (LVEF), and QLA10.0 software was used to track the collected myocardial images and obtain GLS to analyze the myocardial movement. Results　of CRP and ESR in acute phase of KD children were collected and compared with those of 56 healthy children. Correlation between inflammatory indexes and GLS in children with KD was analyzed. Results The levels of GLS, CRP and ESR in children with acute KD were higher than those in healthy controls, and there were significant differences (P all<0.01). There was no difference in LVEF between the two groups (P>0.05). The levels of CRP and ESR in non-coronary artery injury group were significantly higher than those in coronary artery injury group, and there were significant differences (P all<0.01). There was no difference in LVEF and GLS between the two groups (P>0.05). In acute phase of KD, GLS was positively correlated with CRP and ESR (r=0.62, 0.57; P all<0.05). Conclusion　The GLS obtained by STE was significantly elevated in the acute phase of KD and was associated with the increased CRP, but was not related to coronary artery injury.