粪便自诱导分子-2在新生儿坏死性小肠结肠炎临床病情监测中的价值

doi:10.3969/j.issn.1000-3606.2019.08.003

临床儿科杂志 ›› 2019, Vol. 37 ›› Issue (8): 570-.doi: 10.3969/j.issn.1000-3606.2019.08.003

粪便自诱导分子-2在新生儿坏死性小肠结肠炎临床病情监测中的价值

付春燕,　王政力,　肖洒,　艾青,　李禄全

重庆医科大学附属儿童医院新生儿诊治中心　儿童发育疾病研究教育部重点实验室　儿童发育重大疾病国家国际科技合作基地　儿科学重庆市重点实验室国家住院医师规范化培训示范基地 (重庆　400014)

发布日期:2019-08-09
通讯作者: 李禄全，王政力　电子信箱：liluquan123@163.com， doc.wang@foxmail.com
基金资助:
国家自然科学基金（No. 81601323）；重庆市科委科研基金（No. cstc2018jscx-msyb-X0027）；重庆市人社局留创计划项目（ No. Cx2017107）

Significance of fecal autoinducer-2 in monitoring the occurrence and recovery of neonatal necrotizing enterocolitis

FU Chunyan, WANG Zhengli, XIAO Sa, AI Qing, LI Luquan

Neonatal Diagnosis and Treatment Center, Children’s Hospital of Chongqing Medical University; Ministry of Education Key Laboratory of Child Development and Disorders; China International Science and Technology Cooperation Base of Child Development and Critical Disorders; Key Laboratory of Pediatrics in Chongqing; National Demonstration Base of Standardized Training Base for Resident Physicians. Chongqing 400014, China

Published:2019-08-09

摘要/Abstract

摘要： 　目的　初步探讨粪便自诱导分子-2（AI-2）监测新生儿坏死性小肠结肠炎（NEC）病情变化的价值。方法　以 2017年10月至2018年4月收治的12例NEC患儿为研究对象，并按照胎龄、日龄、生产方式、喂养方式、抗生素使用等选择 12例与NEC组患儿匹配的非NEC新生儿为对照组。NEC诊断时定为急性期，再次开奶3天后定为恢复期。采集NEC急性期、恢复期和对照组的粪便标本，以BB170生物荧光检测法检测粪便AI-2浓度，采用16s rDNA 高通量测序分析粪便菌群。结果　在菌门水平，拟杆菌门比例在NEC组急性期、恢复期和对照组之间差异有统计学意义（P<0.05），其中NEC恢复期比例最低。在菌属水平，肠球菌属、拟杆菌属比例在三组之间差异均有统计学意义（P<0.05），其中NEC急性期肠球菌属比例最低，恢复期拟杆菌属比例最低。三组粪便AI-2浓度差异有统计学意义（P<0.05），以NEC急性期为最低。结论　AI-2 在监测NEC病情变化方面具有潜在临床应用价值。

关键词: 自诱导分子-2；　坏死性小肠结肠炎；　肠道菌群；　新生儿

Abstract: Objective　To explore the significance of fecal autoinducer-2 (AI-2) in monitoring the occurrence and recovery of neonatal necrotizing enterocolitis (NEC). Methods　Twelve newborns with NEC, diagnosed between October 2017 to April 2018, were included in the present study (the NEC group, n=12). Another 12 newborns were paired with gestational age, age, way of delivery, way of feeding, use of antibiotics and so on, to the NEC group as control group (n=12). The onset of NEC was defined as acute phase and the recovery phase was recognized from the third day after re-introducing feeding of milk. Fecal samples from control group, NEC acute phase and NEC recovery phase were collected. The AI-2 concentration in feces was detected by biofluorescence assay. The fecal flora was analyzed by 16S rDNA high-throughput sequencing. Results　Intestinal flora in Phylum level, the Bacteroides in the acute phase of NEC was significantly lower than that in the control group (P < 0.05). The Bacteroides abundance of NEC in the recovery phase was further lower than that in the acute phase (P < 0.05). In the generic level, Enterococcus and Bacteroides decreased significantly in acute phase of NEC than that in the control group and the recovery phase of NEC (P < 0.05). There was no significant difference in Shannon index among groups. Relative biofluorescence values of feces AI-2 in acute phase of NEC was lower than those in recovery phase of NEC and control groups (P < 0.05). Conclusion　AI-2 has a strong correlation with the changes of NEC and intestinal flora disorders, which may have potential clinical value in monitoring the occurrence and recovery of NEC.

Key words: 　autoinducer-2;　necrotizing enterocolitis;　intestinal flora;　diversity

付春燕，王政力，肖　洒，等. 粪便自诱导分子-2在新生儿坏死性小肠结肠炎临床病情监测中的价值[J]. 临床儿科杂志, 2019, 37(8): 570-.

FU Chunyan, WANG Zhengli, XIAO Sa, et al. Significance of fecal autoinducer-2 in monitoring the occurrence and recovery of neonatal necrotizing enterocolitis[J]. Journal of Clinical Pediatrics, 2019, 37(8): 570-.

粪便自诱导分子-2在新生儿坏死性小肠结肠炎临床病情监测中的价值

Significance of fecal autoinducer-2 in monitoring the occurrence and recovery of neonatal necrotizing enterocolitis

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