关键词: 新生儿呼吸窘迫综合征；　miR-200c-3p；　预后判断

Abstract: Objective　To explore the value of serum mir-200c-3p expression in determining the severity and prognosis of neonatal respiratory distress syndrome (NRDS). Methods　A total of 102 NRDS newborns admitted to the Second Affiliated Hospital of Hainan Medical College from June 2016 to June 2019 were selected and the Score for Neonatal Acute Physiology with Perinatal Extension-II (SNAPPE II) was calculated. According to the survival of the children, they were divided into the survival group (n=73) and the death group (n=29). According to the X-ray results and the severity of the disease, the children were divided into the mild group (grade Ⅰ and Ⅱ, 67 cases) and the severe group (grade Ⅲ and Ⅳ, 35 cases). The expression level of miR-200c-3p was detected by real-time fluorescent quantitative PCR. ROC curve was used to analyze the miR-200c-3p levels and SNAPPE-II score in predicting death in children with NRDS. Results　The serum miR-200c-3p expression level and SNAPPE-II score in the death group were significantly higher than those in the survival group, the expression level of miR-200c3p, SNAPPE-II score and mortality rate in the severe group were significantly higher than those in the mild group, and differences were statistically significant (P<0.05). ROC curve analysis showed that the area under the curve (AUC) of serum miR-200c-3p expression combined with SNAPPE-II was 0.93 (95%CI: 0.87~0.98) in predicting NRDS death, significantly higher than that of miR-200c-3p alone (0.84, 95%CI: 0.78~0.90) or SNAPPE-II alone (0.80, 95%CI: 0.75~0.86), and differences were statistically significant (P<0.05). Its sensitivity and specificity were 94.0% and 88.5% respectively. There was a significant positive correlation between the expression of miR-200c-3p and SNAPPE-II in NRDS (r= 0.84, P< 0.01). Conclusions　The increased expression of miR-200c-3p in serum is related to the severity and prognosis of NRDS. The combination of miR-200c-3p and SNAPPE-II is of high value in the prognosis of NRDS.

Key words: neonatal respiratory distress syndrome;　miR-200c-3p;　prognostic judgement