苦参碱对人肾母细胞瘤SK-NEP-1细胞趋化因子受体4表达的影响

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (5): 467-470.

苦参碱对人肾母细胞瘤SK-NEP-1细胞趋化因子受体4表达的影响

毛玲¹, 薛天阳², 许伟²

1.徐州医学院（江苏徐州　221004）；2.徐州医学院附属医院儿科（江苏徐州　221002）

收稿日期:2013-12-27 出版日期:2014-05-15 发布日期:2014-05-15

The effect of matrine on CXCR4 expression in SK-NEP-1 cells

MAO Ling, XUE TianYang，XU Wei

（Department of Pediatrics ,The Affiliated Hospital of Xuzhou Medical College，Xuzhou 221002， Jiangsu，China）

Received:2013-12-27 Published:2014-05-15 Online:2014-05-15

摘要/Abstract

摘要：

　目的　探讨苦参碱对体外培养人肾母细胞瘤SK-NEP-1细胞抑制增殖及诱导凋亡的作用。方法　体外培养人肾母细胞瘤SK-NEP-1细胞分对照组，0.5 mg/ml、1.0 mg/ml、1.5 mg/ml苦参碱干预组，应用四甲基偶氮蓝比色法、流式细胞术检测苦参碱对SK-NEP-1细胞抑制率、凋亡率的影响，逆转录聚合酶链反应检测SK-NEP-1细胞的趋化因子受体4（CXCR4） mRNA表达。结果　不同浓度苦参碱干预组及对照组SK-NEP-1细胞的抑制率、凋亡率以及CXCR4 mRNA表达差异均有统计学意义（P<0.01）；随苦参碱浓度增加，SK-NEP-1细胞的抑制率和凋亡率呈上升趋势，而CXCR4 mRNA表达呈下降趋势，差异均有统计学意义（P<0.01）。结论　苦参碱可以抑制SK-NEP-1细胞增殖并诱导凋亡，该作用可能与下调CXCR4的表达有关。

Abstract: 　Objective　To investigate the effects of matrine on the proliferation and apoptosis of SK-NEP-1 cells in vitro,and its possible mechanism. Methods Trials were divided into following groups: control group, 0.5, 1.0 and 1.5mg/ml of matrine intervention groups. The inhibition rate of SK-NEP-1 cells treated with different concentration of Matrine was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry (FCM). RT-PCR analysis was employed to measure the PDCD4 mRNA expression. Results Matrine (final concentrations=0.5, 1.0 and 1.5mg/ml) could induce apoptosis and inhibit the growth of SK-NEP-1 cells. Compared with the controls without matrine treatment (8.81±3.71) %, the inhibition rates of SK-NEP-1 cells were (20.79±6.20)%, (31.25±5.07)%, and (51.15±12.70)%, respectively; the apoptotic rates of SK-NEP-1 cells treated with different concentration of matrine were (13.67±0.78)%,(17.43±1.65)% and (20.80±1.54)%, respectively. Significant difference in the inhibition and apoptotic rates of SK-NEP-1 cells between each drug group and control group was observed(P<0.05), and the inhibition and apoptotic rates of SK-NEP-1 cells increased gradually with increased matrine concentration, thus exhibiting a dose-dependent effect （P<0.05）. To the expression of CXCR4 mRNA，the grey levels of SK-NEP-1 cells treated with matrine intervention group (final concentrations=0.5, 1.0 and 1.5 mg/ml) were (0.720±0.058), (0.540±0.095) and (0.307±0.050), respectively. The mRNA expression of CXCR4 was seen in SK-NEP-1 cells. Compared with control group, the expression of CXCR4 mRNA was decreased significantly in matrine intervention group (P<0.01).There were significant difference in CXCR4 mRNA level among the SK-NEP-1 cells treated with 0.5,1.0,1.5mg/mL of matrine (P<0.01). Conclusion Matrine could induce apoptosis and inhibit the growth of SK-NEP-1 cells in a dose-dependent way which may be associated with the down-regulated CXCR4 expression in SK-NEP-1 cells.

毛玲, 薛天阳, 许伟. 苦参碱对人肾母细胞瘤SK-NEP-1细胞趋化因子受体4表达的影响[J]. 临床儿科杂志, 2014, 32(5): 467-470.

MAO Ling,XUE TianYang,XU Wei. The effect of matrine on CXCR4 expression in SK-NEP-1 cells [J]. , 2014, 32(5): 467-470.

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