CD40信号激活对肾小管上皮细胞的影响

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (1): 62-65.

CD40信号激活对肾小管上皮细胞的影响

陈艳杰² 李晓忠¹ 梅俊² 谷丽²

1. 江苏省苏州市苏州大学附属儿童医院肾内科（江苏苏州　100034）；
2.上海市同济大学附属第十人民医院儿科（上海　200072）

收稿日期:2014-01-15 出版日期:2014-01-15 发布日期:2014-01-15

The effect of CD40 message activation on the renal tubular epithelial cells CHEN Yanjie², LI Xiaozhong¹, MEI Jun², GU Li² (1.Department of Kidney, Soochow University Affiliated Children's Hospital, Suzhou 100034, Jiangsu, China; 2.Department of Pediatrics, The Tenth People's Hospital Affiliated to Tongji University, Shanghai 200072, China)

Received:2014-01-15 Online:2014-01-15 Published:2014-01-15

摘要/Abstract

摘要：

　目的　探讨CD40信号激活对肾小管上皮细胞（HK2）的作用。方法　以不同剂量的CD40激发型单克隆抗体抗（5c11）刺激HK2细胞，以四甲基偶氮唑盐（MTT）法和流式细胞术检测细胞增殖和凋亡，以酶联免疫吸附法检测HK2培养中趋化因子受激活调节正常T细胞表达和分泌因子（RANTES）水平。结果　HK2表面CD40表达高达78.9%。在不同浓度5c11刺激下，第9天时随5c11浓度增加HK2细胞增殖（OD值）逐步下降，各组间的差异均有统计学意义（P<0.01）；第6、第9天时HK2凋亡率升高，差异有统计学意义（P<0.01）；RANTES分泌随5c11浓度增加而升高，差异有统计学意义（P均<0.05），并随时间推移呈增加趋势，差异有统计学意义（P均<0.01）。结论　CD40信号在HK2细胞中的表达在导致肾固有细胞凋亡以及效应性细胞因子的分泌过程中起重要调节作用。

Abstract: 　Objective　To explore the effect of CD40 message activation on the renal tubular epithelial cells (HK2). Methods After HK2 cells are activated by agonistic anti-CD40 monoclonal antibody 5c11 at 5 and 10 μg/ml, their proliferation and apoptosis were tested with MTT and flow cytometry respectively at day 3, 6 and 9. Simultaneously, level of RANTES (regulated upon activation, normal T cell expressed and secreted) in culture supernatant of HK2 cells was measured by ELISA. Results The expression of CD40 in HK2 cells was 78.9%. After activation for 9 days with 5c11 of different concentrations, the proliferation of HK2 cells (OD value) was decreased as 5c11 concentrations increased by MTT test, and the difference among groups was significant (P<0.01). However, the apoptosis rate of HK2 cells was increased at day 6 and day 9 by flow cytometry, and the difference was significant (P<0.01). The level of RANTES was elevated as 5c11 concentrations and time increased, and the differences were significant (P<0.05). Conclusions The expression of CD40 in HK2 cells plays important roles in regulating the proliferation, apoptosis and inflammatory factor release of renal tubular cells.

陈艳杰李晓忠梅俊谷丽. CD40信号激活对肾小管上皮细胞的影响[J]. 临床儿科杂志, 2014, 32(1): 62-65.

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(收稿日期：2013-07-08)
（本文编辑：邹　强）