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SP-B 外显子4(T131I)位点与蒙古族新生儿呼吸窘迫综合征相关性研究

  • 张亚昱 ,
  • 新春 ,
  • 胡亚楠 ,
  • 梅花 ,
  • 王晶 ,
  • 刘春枝 ,
  • 刘春丽
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  • 内蒙古医科大学附属医院新生儿科(内蒙古呼和浩特 010050)

收稿日期: 2016-11-15

  网络出版日期: 2016-11-15

基金资助

国家自然科学基金项目(No. 81260107);内蒙古自然科学基金项目(No. 2011MS1111)

Correlation between the SP-B exon 4 (T 131 I) and neonatal respiratory distress syndrome in Western Inner Mongolia

  • ZHANG Yayu ,
  • XIN Chun ,
  • HU Yanan ,
  • MEI Hua ,
  • WANG Jing ,
  • LIU Chunzhi ,
  • LIU Chunli
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  • Neonatal Paediatrics Ward in the Department of Pediatrics, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, Inner Mongolia, China

Received date: 2016-11-15

  Online published: 2016-11-15

摘要

 目的 探讨SP-B外显子4(T131I)位点与内蒙古西部地区新生儿呼吸窘迫综合征(NRDS)易感性的关系。方 法 采用病例对照研究方法,选择于2009年9月-2016年2月住院诊断为NRDS的蒙古族早产儿86例作为病例组,选择 同期未发生NRDS的蒙古族早产儿86例作为对照组。应用聚合酶链反应-单链构象多态(PCR-SSCP)分析技术及基因测 序技术检测SP-B基因exon4区域上有无突变,以及T131I位点的基因型、等位基因分布。结果 在所有研究对象中,SP-B 基因exon4区域无突变发生,T131I位点基因型均可检出3种基因型,即CC、CT、TT,病例组所占比例分别为58.1%、 27.9%、14.0%,对照组分别为40.7%、43.0%、16.3%,两组基因型分布的差异无统计学意义(χ2=5.57, P=0.062);病例 组C等位基因频率为80.2%,高于对照组(64.0%),差异有统计学意义(χ2=11.33, P < 0.001)。 结论 SP-B基因外显子 4 (T131I)位点基因多态性可能是蒙古族NRDS易感基因之一。

本文引用格式

张亚昱 , 新春 , 胡亚楠 , 梅花 , 王晶 , 刘春枝 , 刘春丽 . SP-B 外显子4(T131I)位点与蒙古族新生儿呼吸窘迫综合征相关性研究[J]. 临床儿科杂志, 2016 , 34(11) : 846 . DOI: 10.3969/j.issn.1000-3606.2016.11.011

Abstract

 Objective To explore the relationship between the SP-B exon 4 (T131I) and susceptibility of neonatal respiratory distress syndrome (NRDS) in Western Inner Mongolia. Methods A case control study from September 2009 to February 2016 in the Affiliated Hospital of Inner Mongolia Medical University neonatal ward was performed. Eighty-six preterm infants of Mongol nationality suffered from NRDS were selected as the case group, while eighty-six preterms in the same period without NRDS were selected as the control group. Polymerase chain reaction were used to detect mutations in exon 4 of SP-B, genotype, and allelic distribution of T131I of exon 4, as well. Results  Newborns in western Inner Mongolia: ①No mutation was found in SP-B exon 4. ②The frequencies of genotype CC, CT and TT of T131I in the NRDS group were 58.1%、27.9% and 14.0%, respectively. Frequency of allele C were 80.2%, and frequency of allele T were 19.8%.  In the control group,?the frequencies of genotype CC, CT and TT were 40.7%、43.0% and 16.3%, respectively. Frequency of allele C were 64.0%, and frequency of allele T were 36.0%, there was significant difference in the frequencies of SP-B exon 4 T131I sites between these two groups (P < 0.05). Conclusion There was no mutation found in SP-B exon 4. Exon 4 (T131I) gene polymorphism of SP-B gene may be one of the susceptible locus of Mongolian infants in the western region of Inner Mongolia.
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