目的 探讨IL-13基因+2044G/A多态性与儿童哮喘发病风险及哮喘儿童血清IL-13水平的关系。方法 采 用PCR-RFLP和ELISA方法，对90例支气管哮喘患儿（哮喘组） 和82例健康儿童（对照组）IL-13基因+2044G/A多 态性和血清IL-13水平进行检测，计算基因型、等位基因频率及其所对应的血清IL-13水平。结果 哮喘组和对照组 IL-13+2044G/A多态性3种基因型的分布差异有统计学意义（P ＜ 0.001）；与GG基因型比较，GA基因型儿童哮喘风险增加 3.52倍（95% CI：1.82~6.82），AA基因哮喘风险增加3.71倍（95% CI：1.06~12.97）。 哮喘组A等位基因频率为36.1%， 高于对照组的18.3%，差异有统计学意义（P ＜ 0.001）。与G等位基因比较， A等位基因携带儿童哮喘风险增加2.53倍（95% CI：1.53~4.16）。 哮喘组患儿血清IL-13水平为151.82（134.33~166.68）ng/L，对照组为79.00（58.74~111.09）ng/L， 两组差异有统计学意义（P ＜ 0.001）。 哮喘组GG、GA和AA基因型的血清IL-13水平均分别高于对照组，差异有统计学 意义（P均 ＜ 0.01）。 哮喘组GG、GA和AA 3种基因型之间血清IL-13水平的差异也有统计学意义（P ＜ 0.01），其中GA和 AA基因型血清IL-13水平高于GG基因型。 结论 IL-13基因+2044G/A多态性与支气管哮喘儿童血清IL-13水平具有关 联性，且A等位基因可能造成了哮喘儿童血清高IL-13水平，携带A等位基因的儿童哮喘风险增加。

Objective To investigate the relationship between IL-13 gene + 2044G / A polymorphism to the risk of asthma in children and its relevancy of serum IL-13 levels. Methods Ninty cases of children with bronchial asthma (asthma group) and 82 healthy children (control group) were recruited. PCR-RFLP and ELISA were used to detect IL-13 gene 2044G?＞?A polymorphism and serum IL-13 levels. Genotype, allele frequencies and the corresponding serum IL-13 level were calculated. χ2 test and t-test were used to compare the genotype, allele frequency, and serum IL-13 level between the two groups. Results The distribution of IL-13 gene 2044G?＞?A polymorphism showed significant difference between the two groups. Compared with AA genotype and A allele, children with GG genotype and G allele were associated with an increased  risk of bronchial asthma, and the OR value were 3.706 (95%CI:1.059~12.976) (P ＜ 0.001) and 2.525 (95%CI:1.532~4.159) (P ＜ 0.001). The level of serum IL-13 is higher in asthma group than in control group (Z = 7.899, P ＜ 0.001), and the serum IL-13 level of GA and AA in asthma group is higher than that in children with  GG genotype (Z = 5.555, P ＜ 0.001; Z = 3.541, P ＜ 0.001; Z = 2.777, P ＜ 0.05). Conclusion There is association between IL-13 2044G?＞?A polymorphism and serum IL-13 level in children' with bronchial asthma. And A allele maybe associated with higher serum IL-13 level in children with asthma.