脂肪乳剂对脂多糖诱导的急性肺损伤中细胞因子IL-1β和IL-6的影响

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (3): 250-253.

脂肪乳剂对脂多糖诱导的急性肺损伤中细胞因子IL-1β和IL-6的影响

石计朋¹, 黄丽密², 钱燕², 尚云^1,

1.新乡医学院第一附属医院新生儿科（河南新乡　453003）；
2.温州医科大学附属第一医院新生儿科（浙江温州　325000）

收稿日期:2013-07-19 出版日期:2014-03-15 发布日期:2014-03-15

Effect of lipid emulsions on the IL-1β and IL-6 expressions in acute lung injury induced by endotoxin in rats

SHI Jipeng¹， HUANG Limi²， QIAN Yan²， SHANG Yun¹

(1. Department of Neonatology， The First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, Henan, China; 2. Department of Neonatology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China)

Received:2013-07-19 Published:2014-03-15 Online:2014-03-15

摘要/Abstract

摘要：

　目的　比较3种脂肪乳剂对脂多糖（LPS）诱导的急性肺损伤（ALI）大鼠IL-1β和IL-6分泌的影响。方法　100只SD幼年大鼠分为对照组、LPS组、ω-9组、ω-6组和ω-3组。日龄28 d时连续7 d，对照组大鼠经尾静脉注射生理盐水；ω-6组注射脂肪乳(C14～24)、ω-9组注射长链脂肪乳、ω-3组注射ω-3鱼油脂肪乳注射液；随后对照组气管内滴入生理盐水，另4组大鼠均喷洒LPS溶液。8 h后处死大鼠观察各组肺组织的病理改变，并测定IL-1β 和IL-6 mRNA及蛋白等水平。结果　各组大鼠肺组织病理切片均可见明显炎症细胞浸润和出血；4组ALI大鼠的肺系数、肺损伤评分均高于对照组，差异均有统计学意义（P均<0.005）；ω-9组和ω-3组两组肺组织中IL-1β和IL-6 mRNA表达均较LPS组和ω-6组降低，差异均有统计学意义（P均<0.005）；支气管肺泡灌洗液中ω-9组和ω-3 组IL-1β和IL-6蛋白水平均低于其余组，差异均有统计学意义（P均<0.005）。ω-9组和ω-3组IL-1β和IL-6 mRNA表达及其相应的蛋白水平差异均无统计学意义（P均>0.005）。结论　ω-6 PUFAs可增加IL-1β和IL-6的产生加重ALI大鼠炎症反应；而ω-9 MUFAs和ω-3 PUFAs可降低IL-1β和IL-6减轻ALI大鼠炎症反应，具有一定的抗炎作用。

Abstract: 　Objective　To compare the effect of three types of lipid emulsions on the IL-1β and IL-6 expressions in acute lung injury induced by lipopolysaccharide (LPS) in rats. Methods One hundred young SD rats were divided into control， LPS， ω-6， ω-9 and ω-3 group. Age 29 days, the control group were intravenously injected with saline, ω-6 group injected lipid emusion (C14-24), ω-9 group injected long chain fat emusion, ω-3 group injected fish oil fat emusion. Then the control group followed by intratracheal instillation of saline, and the reamining four groups were sprayed with LPS. Pathologic changes in lung tissue section were observed. The expression levels of IL-1β mRNA and IL-6 mRNA were determined by RT-PCR， and the concentrations of IL-1β and IL-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immune-specific assay. Results Infiltration and bleeding were observed in lung tissue under light microscopy in ALI rats. The lung indexes and pathological scores of ALI model groups were significantly higher than those of control group (P<0.005). The expressions of IL-1β and IL-6 mRNA in groups ω-9 and ω-3 were significantly higher than those in groups LPS and ω-6 (P<0.005). The levels of IL-1β and IL-6 in BALF in groups ω-9 and ω-3 were significantly higher than those in groups LPS and ω-6 (P<0.005). There was no significant difference (P>0.005) of the expressions of IL-1β and IL-6 mRNA between ω-9 and ω-3 group. Conclusions ω-6 PUFAs can accentuate inflammation by up-regulating the levels of IL-1β and IL-6 while ω-9 PUFAs and ω-3 PUFAs can relieve inflammation by down-regulating the levels of IL-1β and IL-6.

石计朋, 黄丽密, 钱燕, 尚云. 脂肪乳剂对脂多糖诱导的急性肺损伤中细胞因子IL-1β和IL-6的影响[J]. 临床儿科杂志, 2014, 32(3): 250-253.

SHI Jipeng,HUANG Limi,QIAN Yan,SHANG Yun. Effect of lipid emulsions on the IL-1β and IL-6 expressions in acute lung injury induced by endotoxin in rats [J]. , 2014, 32(3): 250-253.

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