地塞米松对肺炎支原体感染大鼠血清及肺泡灌洗液中IL-12、IL-13的影响

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (3): 265-270.

地塞米松对肺炎支原体感染大鼠血清及肺泡灌洗液中IL-12、IL-13的影响

王志华^*, 王玉水，刘艳

天津市南开医院儿科（天津　300100）

收稿日期:2013-07-19 出版日期:2014-03-15 发布日期:2014-03-15

Effects of dexamethasone on the levels of IL-12 and IL-13 in bronchoalveolar lavage fluid and serum in rats

WANG Zhihua, WANG Yushui, LIU Yan

(Department of Pediatrics, Tianjin Nankai Hospital, Tianjin 300100, China)

Received:2013-07-19 Published:2014-03-15 Online:2014-03-15

摘要/Abstract

摘要：

阿奇霉素组、地塞米松组、联合干预组和模型对照组，每组各25只。各组大鼠均予MP菌液（1×10⁶ CFU/ml）缓慢滴入大鼠鼻腔，共4 d，在最后1次接种后第2天开始干预。采用ELISA方法监测干预后第3、5、8天大鼠血清及BALF液IL-12、IL-13水平，并对肺组织炎症浸润程度进行评分。结果　在干预第3、5、8天，阿奇霉素组、地塞米松组、联合干预组及模型对照组肺组织病理评分比较差异均有统计学意义（F=8.71~20.11，P均<0.01），其中均以联合干预组评分最低。在干预第3、5、8天，四组间血清及BALF液中IL-12、IL-13水平比较差异均有统计学意义（F=9.52~143.47，P均<0.01）；其中干预第3天，联合干预组血清IL-12高于阿奇霉素组，而血清及BALF液IL-13均低于阿奇霉素组，差异均有统计学意义（P均<0.05）。结论　地塞米松可能协同阿奇霉素通过促进大鼠体内IL-12分泌和抑制IL-13表达，减轻肺组织炎症。

王志华, 王玉水，刘艳. 地塞米松对肺炎支原体感染大鼠血清及肺泡灌洗液中IL-12、IL-13的影响[J]. 临床儿科杂志, 2014, 32(3): 265-270.

WANG Zhihua,WANG Yushui,LIU Yan . Effects of dexamethasone on the levels of IL-12 and IL-13 in bronchoalveolar lavage fluid and serum in rats [J]. , 2014, 32(3): 265-270.

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