健康儿童睁闭眼不同状态下多频段大脑磁活动研究

doi:10.12372/jcp.2022.21e0872

摘要/Abstract

摘要：

目的探讨健康儿童睁眼和闭眼不同状态下的多频段大脑磁活动特征。方法记录24名健康儿童睁眼和闭眼时的脑磁图数据。应用累积源成像方法,对大脑磁活动在多个频段进行分析,包括δ（1~4 Hz）、 θ（4~8 Hz）、α（8~12 Hz）、β（12~30 Hz）、低γ（30~55 Hz）和高γ（65~90 Hz）频段。结果纳入研究24名,平均年龄（10.3±2.4）岁,男14名、女10名。闭眼状态组儿童的α和β频段脑磁源绝对强度较睁眼状态组显著增加,差异有统计学意义（P<0.01）。与睁眼状态组相比,闭眼状态组儿童α和β频段脑磁源相对强度显著升高,差异有统计学意义（P<0.01）。闭眼状态组的δ、低γ和高γ频段脑磁源相对强度较睁眼状态组显著降低,差异有统计学意义（P<0.05）。各频段的脑磁源位置分布在睁、闭眼状态下差异无统计学意义（P>0.05）。结论睁眼和闭眼状态下,大脑活动在较宽的频率范围内发生显著变化。闭眼可以调节多种频率范围内的大脑生理活动。在脑磁图或脑电图的研究中,应考虑睁眼闭眼的基线条件差异。

关键词: 脑磁图, 累积源成像, 多频分析, 睁眼, 闭眼

Abstract:

Objective To investigate the characteristics of brain activities within multi-frequency ranges in the developing brain during eyes-open and eyes-closed. Methods The magnetoencephalography (MEG) data were recorded from 24 healthy children during eyes-open and eyes-closed. The MEG sources were localized with accumulated source imaging and analyzed in multi-frequency bands, including delta (1-4 Hz), theta (4-8 Hz), alpha (8-12 Hz), beta (12-30 Hz), low-gamma (30-55 Hz) and high-gamma (65-90 Hz). Results Twenty-four subjects (14 boys and 10 girls) were enrolled, and the average age was (10.3±2.4) years. Compared with eyes-open, the absolute source power of alpha and beta activities during eyes-closed was increased in children, and the difference was statistically significant (P<0.01). Compared with eyes-open, the relative source power of alpha and beta activities was increased (P<0.01), while the relative source power of delta, low-gamma and high-gamma activities was decreased during eyes-closed in children, and the differences were statistically significant (P<0.05). There was no significant difference in the source location distribution of each frequency band between eyes-open and eyes-closed in children (P>0.05). Conclusions The brain activity varies significantly over a wide frequency range between eyes-open and eyes-closed in children. Eye closure can regulate brain physiological activity in a wide range of frequencies. The differences in baseline conditions between eyes-closed and eyes-open should be considered in MEG or EEG studies.

Key words: magnetoencephalography, accumulated source imaging, multi-frequency analysis, eyes-open, eyes-closed

范玉颖, 向敬, 刘雪雁, 王华. 健康儿童睁闭眼不同状态下多频段大脑磁活动研究[J]. 临床儿科杂志, 2022, 40(3): 202-207.

FAN Yuying, XIANG Jing, LIU Xueyan, WANG Hua. A study of multi-frequency neuromagnetic brain activities in children with eyes-open and eyes-closed[J]. Journal of Clinical Pediatrics, 2022, 40(3): 202-207.

图/表 4

表1

表2

表3

图1

参考文献 22

[1]	Ritter SM, Abbing J, van Schie HT. Eye-closure enhances creative performance on divergent and convergent creativity tasks[J]. Front Psychol, 2018, 9: 1315. doi: 10.3389/fpsyg.2018.01315
[2]	Wostmann M, Schmitt LM, Obleser J. Does closing the eyes enhance auditory attention? eye closure increases attentional alpha-power modulation but not listening performance[J]. J Cogn Neurosci, 2020, 32(2): 212-225. doi: 10.1162/jocn_a_01403
[3]	Ishii R, Canuet L, Aoki Y, et al. Frequency diversity of posterior oscillatory activity in human revealed by spatial filtered MEG[J]. J Integr Neurosci, 2013, 12(3): 343-353. doi: 10.1142/S0219635213500209
[4]	Cragg L, Kovacevic N, McIntosh AR, et al. Maturation of EEG power spectra in early adolescence: a longitudinal study[J]. Dev Sci, 2011, 14(5): 935-943. doi: 10.1111/j.1467-7687.2010.01031.x pmid: 21884309
[5]	Saby JN, Marshall PJ. The utility of EEG band power analysis in the study of infancy and early childhood[J]. Dev Neuropsychol, 2012, 37(3): 253-273. doi: 10.1080/87565641.2011.614663
[6]	Rodriguez-Martinez EI, Ruiz-Martinez FJ, Barriga Paulino CI, et al. Frequency shift in topography of spontaneous brain rhythms from childhood to adulthood[J]. Cogn Neurodyn, 2017, 11(1): 23-33. doi: 10.1007/s11571-016-9402-4
[7]	Xiang J, Luo Q, Kotecha R, et al. Accumulated source imaging of brain activity with both low and high-frequency neuromagnetic signals[J]. Front Neuroinform, 2014, 8: 57. doi: 10.3389/fninf.2014.00057 pmid: 24904402
[8]	Xiang J, Wang Y, Chen Y, et al. Noninvasive localization of epileptogenic zones with ictal high-frequency neuromagnetic signals[J]. J Neurosurg Pediatr, 2010, 5(1): 113-122. doi: 10.3171/2009.8.PEDS09345
[9]	Xiang J, Liu Y, Wang Y, et al. Neuromagnetic correlates of developmental changes in endogenous high-frequency brain oscillations in children: a wavelet-based beamformer study[J]. Brain Res, 2009, 1274: 28-39. doi: 10.1016/j.brainres.2009.03.068 pmid: 19362072
[10]	Markovska-Simoska S, Pop-Jordanova N. Quantitative EEG in children and adults with attention deficit hyperactivity disorder: comparison of absolute and relative power spectra and theta/beta ratio[J]. Clin EEG Neurosci, 2017, 48(1): 20-32. pmid: 27170672
[11]	Boersma M, de Bie HM, Oostrom KJ, et al. Resting-state oscillatory activity in children born small for gestational age: an MEG study[J]. Front Hum Neurosci, 2013, 7: 600. doi: 10.3389/fnhum.2013.00600 pmid: 24068993
[12]	Lin YT, Lee WC. Importance of presenting the variability of the false discovery rate control[J]. BMC Genet, 2015, 16: 97. doi: 10.1186/s12863-015-0259-z
[13]	Barry RJ, Clarke AR, Johnstone SJ, et al. EEG differences between eyes-closed and eyes-open resting conditions[J]. Clin Neurophysiol, 2007, 118(12): 2765-2773. doi: 10.1016/j.clinph.2007.07.028
[14]	Barry RJ, Clarke AR, Johnstone SJ, et al. EEG differences in children between eyes-closed and eyes-open resting conditions[J]. Clin Neurophysiol, 2009, 120(10): 1806-1811. doi: 10.1016/j.clinph.2009.08.006
[15]	Van't Ent D, van Soelen IL, Stam CJ, et al. Strong resemblance in the amplitude of oscillatory brain activity in monozygotic twins is not caused by "trivial" similarities in the composition of the skull[J]. Hum Brain Mapp, 2009, 30(7): 2142-2145. doi: 10.1002/hbm.v30:7
[16]	Geller AS, Burke JF, Sperling MR, et al. Eye closure causes widespread low-frequency power increase and focal gamma attenuation in the human electrocorticogram[J]. Clin Neurophysiol, 2014, 125(9): 1764-1773. doi: 10.1016/j.clinph.2014.01.021 pmid: 24631141
[17]	Boto E, Seedat ZA, Holmes N, et al. Wearable neuroimaging: Combining and contrasting magnetoence-phalography and electroencephalography[J]. Neuro Image, 2019, 201: 116099.
[18]	Fan Y, Dong L, Liu X, et al. Recent advances in the noninvasive detection of high-frequency oscillations in the human brain[J]. Rev Neurosci, 2020; 32(3): 305-321. doi: 10.1515/revneuro-2020-0073
[19]	Bartoli E, Bosking W, Chen Y, et al. Functionally distinct gamma range activity revealed by stimulus tuning in human visual cortex[J]. Curr Biol, 2019, 29(20): 3345-3358. doi: 10.1016/j.cub.2019.08.004
[20]	Bartoli E, Bosking W, Foster BL. Seeing visual gamma oscillations in a new light[J]. Trends Cogn Sci, 2020, 24(7): 501-503. doi: 10.1016/j.tics.2020.03.009
[21]	Parker A, Dagnall N. Eye-closure & the retrieval of item-specific information in recognition memory[J]. Conscious Cogn, 2020, 77: 102858. doi: 10.1016/j.concog.2019.102858
[22]	Xiang J, Tenney JR, Korman AM, et al. Quantification of interictal neuromagnetic activity in absence epilepsy with accumulated source imaging[J]. Brain Topogr, 2015, 28(6): 904-914. doi: 10.1007/s10548-014-0411-5 pmid: 25359158

频段	闭眼状态组	睁眼状态组	Z值	P
δ	2.626(1.905~3.474)	2.459(1.571~2.961)	1.51	0.130
θ	0.742(0.512~1.554)	0.781(0.440~1.258)	1.54	0.123
α	1.611(1.099~2.505)	0.663(0.435~0.968)	4.29	<0.001
β	2.009(1.515~2.094)	1.098(0.804~1.264)	4.26	<0.001
低γ	0.469(0.360~0.628)	0.417(0.296~0.560)	0.91	0.361
高γ	0.027(0.010~0.065)	0.019(0.009~0.075)	0.26	0.797

频段	闭眼状态组	睁眼状态组	Z值	P
δ	0.309(0.239~0.375)	0.398(0.297~0.459)	3.32	0.001
θ	0.115(0.071~0.144)	0.115(0.086~0.167)	1.26	0.209
α	0.223(0.141~0.317)	0.119(0.087~0.153)	4.29	<0.001
β	0.223(0.194~0.267)	0.136(0.176~0.218)	3.23	0.001
低γ	0.057(0.040~0.073)	0.065(0.048~0.115)	2.57	0.010
高γ	0.003(0.001~0.005)	0.004(0.002~0.008)	2.09	0.037

频段	脑磁源位置	闭眼状态对应数目	睁眼状态对应数目	P¹⁾
δ	脑深部区	11（45.8）	12（50.0）	1.000
	下内侧额叶皮质区	7（29.2）	7（29.2）
	楔前叶皮质区	6（25.0）	5（20.8）
θ	脑深部区	11（45.8）	12（50.0）	0.369
	下内侧额叶皮质区	6（25.0）	9（37.5）
	内侧枕叶皮质区	7（29.2）	3（12.5）
α	内侧枕叶皮质区	24（100.0）	20（83.3）	0.109
	脑深部区	0（0.0）	3（12.5）
	楔前叶皮质区	0（0.0）	1（4.2）
β	楔前叶皮质区	13（54.2）	12（50.0）	0.634
	内侧枕叶皮质区	8（33.3）	6（25.0）
	下内侧额叶皮质区	3（12.5）	6（25.0）
低γ	楔前叶皮质区	13（54.2）	11（45.8）	0.928
	缘上回区	6（25.0）	6（25.0）
	下内侧额叶皮质区	5（20.8）	7（29.2）
高γ	楔前叶皮质区	12（50.0）	11（45.8）	1.000
	缘上回区	7（29.2）	7（29.2）
	下内侧额叶皮质区	5（20.8）	6（25.0）