慢性乙型肝炎患儿外周血MAIT细胞的免疫生物学特性

doi:10.12372/jcp.2024.23e0751

摘要/Abstract

摘要：

目的探讨慢性乙型肝炎患儿外周血黏膜相关恒定T（MAIT）细胞的变化及其临床意义。方法慢性乙型肝炎 (CHB) 组20例，健康对照（HC）组20例，收集患儿外周血，流式细胞技术检测MAIT细胞（CD3⁺CD161⁺TCRVα7.2⁺细胞）及其不同亚型（CD4⁺CD8^－MAIT细胞、CD4^－CD8^－MAIT细胞、CD4^－CD8⁺MAIT细胞和CD4⁺CD8⁺MAIT细胞）比例，表达PD-1、CD69、穿孔素、CD107α、CXCR3、CXCR6和CCR6的MAIT细胞比例，计量资料行Mann-Whitney U检验和Spearman相关性分析。结果与HC相比，CHB组患儿外周血MAIT细胞及其不同亚型比例无变化（P>0.05），外周血表达PD-1、CD69、CD107α、CXCR3、CXCR6、CCR6和穿孔素的MAIT细胞比例均有升高（P<0.05）。表达CD69、PD-1、CD107α、穿孔素、CXCR6、CCR6和CXCR3的MAIT细胞比例与丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）及HBV-DNA荧光定量间无相关性（P>0.5）。结论外周血MAIT细胞的不同免疫功能在儿童慢性乙型肝炎发病中起着一定作用。

关键词: 黏膜相关恒定T细胞, 慢性乙型肝炎, 免疫, 儿童

Abstract:

Objective This study aimed to investigate the changes and the clinical implications of peripheral blood mucosal associated invariant T (MAIT) cells in children with chronic hepatitis B (CHB). Methods The investigation encompassed 20 patients with CHB and an equal number of age-matched healthy controls (HC). Peripheral blood specimens were obtained and analyzed using flow cytometry to quantify the presence of MAIT cells (defined as CD3⁺CD161⁺TCRVα7.2⁺), their subtypes, and the expression of various markers including PD-1, CD69, perforin, CD107α, CXCR3, CXCR6, and CCR6. Statistical comparisons between the groups were made using the Mann-Whitney U test and Spearman's correlation analysis. Results No significant differences were observed in the proportion of MAIT cells and their subtypes between the CHB and HC groups (P>0.05). However, the CHB group exhibited a significant increase in the proportion of MAIT cells expressing PD-1, CD69, CD107α, CXCR3, CXCR6, CCR6, and perforin (P<0.05). There was no significant correlation between the proportion of MAIT cells expressing CD69, PD-1, CD107α, perforin, CXCR6, CCR6 and CXCR3 and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatitis B virus (HBV) DNA fluorescent quantitation (P>0.05). Conclusion The distinct immunological functions of peripheral blood MAIT cells may contribute to the pathogenesis of CHB in pediatric patients.

Key words: mucosal associated invariant T cell, chronic hepatitis B, immunity, child

姜涛, 李双杰, 唐莲, 欧阳文献. 慢性乙型肝炎患儿外周血MAIT细胞的免疫生物学特性[J]. 临床儿科杂志, 2024, 42(9): 787-790.

JIANG Tao, LI Shuangjie, TANG Lian, OUYANG Wenxian. Immunobiological properties of peripheral blood MAIT cells in children with chronic hepatitis B[J]. Journal of Clinical Pediatrics, 2024, 42(9): 787-790.

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参考文献 16

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项目	CHB组	HC组	Z值	P
MAIT细胞	0.59（0.25~1.66）	0.29（0.09~0.69）	1.50	0.134
CD4^－CD8⁺MAIT细胞	53.10(17.90~64.35)	43.65(29.65~64.98)	0.26	0.797
CD4⁺CD8⁺MAIT细胞	12.40(8.21~18.35)	9.36(6.04~22.45)	0.49	0.626
CD4⁺CD8^－MAIT细胞	1.05(0.00~2.55)	0.38(0.00~1.55)	1.24	0.215
CD4^－CD8^－MAIT细胞	28.65(23.45~55.20)	31.25(24.05~50.33)	0.19	0.850
CD69⁺MAIT细胞	32.55(24.00~55.33)	18.9(13.23~31.18)	2.60	0.009
CD107α⁺MAIT细胞	28.48(16.48~50.35)	10.95(6.80~17.18)	3.32	0.001
CD186（CXCR6）⁺MAIT细胞	21.72(10.80~29.80)	6.02(3.27~21.58)	2.92	0.003
CD196(CCR6)⁺MAIT细胞	17.66(11.49~21.91)	4.94(3.32~17.13)	2.65	0.008
CD279(PD-1)⁺MAIT细胞	17.51(13.15~25.09)	4.43(2.07~11.29)	3.49	<0.001
CXCR3⁺MAIT细胞	55.85(45.15~64.10)	44.80(32.93~53.00)	2.39	0.017
穿孔素⁺MAIT细胞	17.04(11.00~22.82)	4.16(1.35~9.53)	3.06	0.002

项目	ALT	AST	HBV-DNA
CD69⁺MAIT细胞	－0.071/0.767	－0.042/0.860	－0.111/0.640
PD-1⁺MAIT细胞	－0.146/0.539	0.113/0.636	－0.361/0.118
CD107α⁺MAIT细胞	0.012/0.960	0.198/0.403	－0.385/0.093
穿孔素⁺MAIT细胞	0.29/0.214	0.136/0.567	0.127/0.593
CXCR6⁺MAIT细胞	－0.235/0.319	－0.323/0.164	－1.08/0.650
CCR6⁺MAIT细胞	0.123/0.607	0.181/0.444	0.261/0.266
CXCR3⁺MAIT细胞	－0.379/0.099	－0.144/0.544	－0.223/0.346