单细胞测序技术在川崎病研究中的应用现状

doi:10.12372/jcp.2023.23e0436

摘要/Abstract

摘要：

川崎病（KD）是一种主要累及全身中小血管的急性发热性疾病，好发于5岁以下儿童，冠状动脉病变是其主要并发症。目前KD已成为发达国家儿童获得性心脏病最常见的原因。不同于传统测序方法，单细胞测序技术在单细胞分辨率下对遗传信息进行测序，不仅能很好地解决细胞异质性问题，还能鉴定细胞亚群、寻找生物标志物、绘制细胞谱系。文章介绍了单细胞测序技术的发展及其在KD研究领域的应用现状，并对其发展前景进行展望。

关键词: 单细胞测序技术, 川崎病, 细胞谱系

Abstract:

Kawasaki disease (KD) is an acute febrile illness which mainly involves the small and medium-sized blood vessels throughout the body, predominantly occurs in children under 5 years old. Coronary artery lesions are its main complication and KD is now the commonest cause of acquired heart disease in children in developed countries. Different from traditional sequencing methods, single-cell sequencing technology can sequence genetic information at single-cell resolution, which can not only solve the problem of cell heterogeneity, but also identify cell subsets, search for biomarkers, and map cell lineages. This commentary will describe the development of single cell sequencing technology and its current application in the field of KD, and provide an outlook on its development.

Key words: single cell sequencing, Kawasaki disease, cell lineage

黄敏. 单细胞测序技术在川崎病研究中的应用现状[J]. 临床儿科杂志, 2023, 41(7): 481-485.

HUANG Min. Application of single cell sequencing technology in Kawasaki disease research[J]. Journal of Clinical Pediatrics, 2023, 41(7): 481-485.

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